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For transfection experiments, cells were seeded into six-well plates overnight and were transiently transfected using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions

467 ZhaoW.XieW.XiaoQ.BeersD.AppelS

Glutathione Conjugation in Xenobiotic Metabolism and in the Production of Intrinsic Bioactive Compounds The conjugation of xenobiotics with GSH is catalyzed by glutathione S-transferase (GST), and, along with glucuronidation and sulfation, this process constitutes a major detoxification system in the liver and kidney [12,18]

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Shwe TT, Yamamoto S, Kakeyama M, Kobayashi T, Fujimaki H